Sysmex公司XN-1000v全自动动物血液分析仪在鱼类上的应用
Abstract
Hematological studies provide essential information about the health of animals, which is crucial for veterinary medicine, scientific research, and aquaculture. Automatic hematological analyzers are an alternative to manual methods, offering faster and more reliable results. The objective of this study was to validate the Sysmex XN-1000V automatic hematology analyzer for blood samples from rainbow trout (Oncorhynchus mykiss), examine the effects of two anticoagulants (K2EDTA and lithium heparin), and establish normal blood reference values for this fish species. Additionally, comparative studies were conducted between the Sysmex XN-1000V and manual methods (hemocytometer cell count and blood smear estimation), and reference intervals were established. Ninety-nine heparinized blood samples were analyzed for validation and sample stability tests. The results showed extremely good precision, with a coefficient of variation (CV) below 3% for RBCs, HGB, and HCT and less than 5% for non-RBC cells (leukocytes plus thrombocytes). However, heterophils (%) exhibited higher variability, with a CV of 15.08%. Linearity was excellent, and the carry-over was below 1% for all parameters. The sample stability test indicated that samples could be analyzed for up to 48 h when stored at 4 ◦C and up to 24 h at room temperature. Non-RBC cells were the first to degrade over time. The automated and manual methods demonstrated good correlation and agreement, validating the analyzer’s accuracy. The effects of two anticoagulants, K2EDTA and lithium heparin, on the blood samples were also studied. Heparin was the preferred anticoagulant for routine hematological analysis of rainbow trout blood with the Sysmex XN-1000V analyzer. In conclusion, the Sysmex XN-1000V enables complete hemogram analyses to be performed quickly and accurately, standardizing techniques, harmonizing results, and providing reliable reference intervals with O mykiss blood.
摘要：
血液学研究提供了有关动物健康的重要信息，这对兽医、科学研究和水产养殖至关重要。自动血液分析仪是手动方法的替代品，可提供更快、更可靠的结果。本研究的目的是验证Sysmex XN-1000V全自动血液分析仪对虹鳟（Oncorhynchus mykiss）血液样本的检测效果，检查两种抗凝剂（K2EDTA和肝素锂）的效果，并为该鱼类建立正常的血液参考值。此外，对Sysmex XN-1000V和手动方法（血细胞计数器细胞计数和血涂片估计）进行了比较研究，并建立了参考区间。对99份肝素化血液样本进行了验证和样本稳定性测试分析。结果显示，精确度极高，红细胞、血红蛋白和红细胞压积的变异系数（CV）低于3%，非红细胞（白细胞和血小板）的变异系数低于5%。然而，异质性（%）表现出更高的变异性，变异系数为15.08%。线性非常好，所有参数的结转率均低于1%。样品稳定性测试表明，在4℃下储存时，样品可以分析长达48小时◦在室温下持续24小时。非红细胞是第一个随着时间的推移而降解的细胞。自动和手动方法显示出良好的相关性和一致性，验证了分析仪的准确性。还研究了两种抗凝剂K2EDTA和肝素锂对血液样本的影响。肝素是Sysmex XN-1000V分析仪对虹鳟血进行常规血液学分析的首选抗凝剂。总之，Sysmex XN-1000V能够快速准确地进行完整的血象分析，规范技术，协调结果，并为O mykiss血液提供可靠的参考区间。
关键词：XN-1000v全自动动物血液分析仪，鱼类健康；血液学；血液学方法验证；血液自动分析仪；虹鳟鱼
