标题：Loligo论文：急性低温胁迫对克氏原螯虾呼吸代谢、抗氧化剂和代谢组学的影响
Abstract
Crayfish (Procambarus clarkii) aquaculture is threatened by abrupt temperature decreases caused by climatic phenomena, such as cold waves and seasonal fluctuations. In this study, crayfish were exposed to an abrupt temperature change from 17 °C to 7 °C for 24 h to investigate the effects of acute low-temperatures on respiratory metabolism, antioxidants, and metabolomics. The results showed that acute low-temperatures significantly reduced the activities of pyruvate kinase, lactate dehydrogenase, and succinate dehydrogenase in the gills and hemolymph, associated with decreases in anaerobic and aerobic respiratory capacities, and significant decreases in oxygen consumption, ammonia excretion, and maximum metabolic rates. Antioxidant enzymes in the hepatopancreas and hemolymph initially increased then decreased within 24 h. Metabolomics revealed that glycerophospholipid metabolism and glycosylphosphatidylinositol anchor biosynthesis pathways responded to acute low-temperatures, with glycerophospholipids being the most significantly differentially expressed metabolites. These results supported the hypothesis that crayfish exhibit lower metabolic activity at low temperatures. Our data provide mechanistic insight into the biological changes induced by acute low-temperature and may provide insight into culture of P. clarkii in cold waters.
摘要：
克氏原螯虾（Procambarus clarkii）的养殖受到寒潮和季节波动等气候现象引起的气温骤降的威胁。在这项研究中，小龙虾暴露在17°C至7°C的突然温度变化中24小时，以研究急性低温对呼吸代谢、抗氧化剂和代谢组学的影响。结果表明，急性低温显著降低了鳃和血淋巴中丙酮酸激酶、乳酸脱氢酶和琥珀酸脱氢酶的活性，这与无氧和有氧呼吸能力的降低以及耗氧量、氨排泄量和最大代谢率的显著降低有关。肝胰腺和血淋巴中的抗氧化酶在24小时内最初增加然后减少。代谢组学显示，甘油磷脂代谢和糖基磷脂酰肌醇锚定生物合成途径对急性低温有反应，其中甘油磷脂是表达差异最大的代谢物。这些结果支持了小龙虾在低温下表现出较低代谢活性的假设。我们的数据为急性低温引起的生物变化提供了机理上的见解，并可能为克拉克氏疟原虫在冷水中的培养提供见解。
关键词：呼吸代谢 Respirometry，Loligo 呼吸代谢，抗氧化剂，代谢组学
